Journal: eLife
Article Title: Gut microbe-derived trimethylamine shapes circadian rhythms through the host receptor TAAR5
doi: 10.7554/eLife.107037
Figure Lengend Snippet: ( A ) Male or female wild-type mice ( Taar5 +/+ ) or mice lacking the TMA receptor ( Taar5 -/ ) were placed in individual cages for indirect calorimetry measured using the Oxymax CLAMS home cage system. After 2 days of equilibration, mice were maintained at thermoneutrality (30°C), room temperature (22°C), or cold stressed (4°C) over a 24-hr period at each temperature point. Oxygen consumption was quantified throughout these temperature transitions as described in the Methods section. ( B ) Male or female wild-type mice ( Taar5 +/+ ) or mice lacking the TMA receptor ( Taar5 -/ ) were necropsied at the beginning of the light cycle (ZT2) or the beginning of the dark cycle (ZT14) and subscapular brown adipose tissue (BAT) was harvested to examine gene. The relative gene expression for circadian genes ( Bmal1l , Nr1d1 , Cry1 , and Per1 ) was quantified by qPCR using the ΔΔ-CT method. Data represent the mean ± SEM from n = 5–9 per group. Significant differences between Taar5 +/+ and Taar5 -/- mice were determined by Student’s t -tests within each individual time point (*p < 0.05).
Article Snippet: Other , OxyMax Clams Home Cage System , Columbus Instruments , NA , Behavioral testing.
Techniques: Gene Expression