Journal: bioRxiv
Article Title: Orai1-mediated Ca 2+ Entry Regulates Lipolysis and Mitochondrial Activation in Brown Adipose Thermogenesis
doi: 10.64898/2026.04.24.718619
Figure Lengend Snippet: a Core body temperature recording in control and Orai1 BKO mice subjected to acute cold exposure (4 °C) for 6 hrs. b infrared thermography images upon cold exposure. c indirect calorimetry during cold exposure (n=6 mice per group). d Gross images of BAT of control and Orai1 BKO under room temperature and cold conditions. e H&E staining of BAT. f qRT-PCR of thermogenic gene expression in BAT. g Western blot analyses of lipolytic and thermogenic proteins. Data are presented as mean ± SEM. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: Indirect calorimetry (O 2 consumption, CO 2 production, RER, and heat production) was assessed using a Comprehensive Lab Animal Monitoring System (Columbus Instruments).
Techniques: Control, Staining, Quantitative RT-PCR, Gene Expression, Western Blot